鳥嘌呤核苷酸交換因子4應(yīng)用說明

中文名稱    鳥嘌呤核苷酸交換因子4    

英文名稱    Epac2

別    名    CAMP GEFII; cAMP regulated guanine nucleotide exchange factor II; cAMP-GEFII; cAMP-regulated guanine nucleotide exchange factor II; CAMPGEFII; CGEF 2; CGEF2; Epac 2; EPAC2; Exchange factor directly activated by cAMP 2; Exchange protein directly activated by cAMP 2; Nbla00496; Putative protein product of Nbla00496; Rap guanine nucleotide exchange factor (GEF) 4; RAP guanine nucleotide exchange factor 4; RAPGEF 4; RAPGEF4; RPGF4_HUMAN.    

供 應(yīng) 商    遠慕生物

研究領(lǐng)域    細胞生物  神經(jīng)生物學(xué)  信號轉(zhuǎn)導(dǎo)      

抗體來源    Rabbit    

克隆類型    Polyclonal    

交叉反應(yīng)    Human, Mouse, Rat, Dog, Cow, Horse, Rabbit,     

產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 鳥嘌呤核苷酸交換因子4（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    116kDa    

細胞定位    細胞漿 細胞膜     

性    狀    Lyophilized or Liquid    

濃    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human Epac2/RAPGEF4    

亞    型    IgG    

純化方法    affinity purified by Protein A    

儲 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

鳥嘌呤核苷酸交換因子4產(chǎn)品介紹    background:

3',5' cyclic adenosine monophosphate (cAMP)-regulated guanine nucleotide exchange factors Epac1 (cAMP-GEFI) and Epac2 (cAMP-GEFII) activate the ras family GTPases Rap1 and Rap2 by promoting GTP binding in a cAMP-dependent manner (1,2). Eukaryotic cAMP is a second messenger that induces physiological responses such as gene expression, growth, differentiation, secretion and neurotransmission (3). Human EPAC2 contains at least 31 exons and maps to chromosome 2q31 (4). The 4.4-kb Epac2 transcript is prominent in brain and adrenal gland (5,6). Within the brain, expression is strong in cortex, occipital pole, frontal lobe, temporal lobe, amygdala, putamen, hippocampus and cerebellum (5,6).

Function:
Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.

Subunit:
Interacts with RIMS1 and RIMS2. Probably part of a complex with RIMS2 and GTP-activated RAB3A (By similarity).

Subcellular Location:
Cytoplasm. Membrane.

Tissue Specificity:
Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels.

Similarity:
Contains 2 cyclic nucleotide-binding domains. Contains 1 DEP domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 Ras-GEF domain.