核苷轉(zhuǎn)運(yùn)蛋白ENT1抗體使用說明書

中文名稱    核苷轉(zhuǎn)運(yùn)蛋白ENT1抗體    

英文名稱    ENT1

別    名    Equilibrative NBMPR-sensitive nucleoside transporter; equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter; Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; Equilibrative nucleoside transporter 1; es-type; MGC1465; MGC3778; Nucleoside transporter; Nucleoside transporter, es-type; OTTHUMP00000016506; OTTHUMP00000016507; OTTHUMP00000016508; OTTHUMP00000016509; OTTHUMP00000016510; OTTHUMP00000016511; OTTHUMP00000016512; S29A1_HUMAN; SLC29A1; solute carrier family 29 (nucleoside transporters), member 1; Solute carrier family 29 member 1.    

供 應(yīng) 商    遠(yuǎn)慕生物    

研究領(lǐng)域    腫瘤  細(xì)胞生物  信號(hào)轉(zhuǎn)導(dǎo)  新陳代謝      

抗體來源    Rabbit    

克隆類型    Polyclonal    

交叉反應(yīng)    Human, Mouse, Rat, Cow, Horse, Sheep, Cynomolgus Monkey, Orangutan    

產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 核苷轉(zhuǎn)運(yùn)蛋白ENT1抗體（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    50kDa    

細(xì)胞定位    細(xì)胞膜     

性    狀    Lyophilized or Liquid    

濃    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human ENT1/SLC29A1    

亞    型    IgG    

純化方法    affinity purified by Protein A    

儲(chǔ) 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

核苷轉(zhuǎn)運(yùn)蛋白ENT1抗體產(chǎn)品介紹    background:

Equilibrative nucleoside transporters (ENTs) regulate many physiological processes and are widely distributed in mammals, plants, yeasts, insects, nematodes and protozoans. They enable facilitated diffusion of hydrophilic nucleosides, such as adenosine and nucleoside analogs, across cell membranes. ENTs are required for uptake of antiviral and anticancer nucleoside drugs and influence a variety of physiological processes, such as neurotransmission and platelet aggregation, by regulating the amount of adenoside available to cell surface receptors. Equilibrative nucleoside transporter 1 (ENT1), also designated solute carrier family 29 (nucleoside transporters), member 1, belongs to the SLC29A transporter family and is a mammalian ENT isoform. ENT1, along with ENT3, mediates the majority of influx and efflux of nucleosides across the plasma membrane.

Function:
Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).

Subcellular Location:
Basolateral cell membrane. Apical cell membrane. Predominantly localized in the basolateral membrane in polarised MDCK cells.

Tissue Specificity:
Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.

Post-translational modifications:
Glycosylated.

Similarity:
Belongs to the SLC29A transporter family.