內(nèi)質(zhì)網(wǎng)氧化物蛋白Ero1-Lα抗體說明書

中文名稱    內(nèi)質(zhì)網(wǎng)氧化物蛋白Ero1-Lα抗體    

英文名稱    ERO1L    

別    名    Endoplasmic oxidoreductin 1 like protein; Endoplasmic oxidoreductin-1-like protein; ERO1 alpha; ERO1 L; ERO1 Lalpha; ERO1 like protein alpha; ERO1-L; ERO1-L-alpha; ERO1-like (S. cerevisiae); ERO1-like protein alpha; ERO1A; ERO1A_HUMAN; ERO1L; Oxidoreductin 1 Lalpha; Oxidoreductin-1-L-alpha.    

供 應 商    遠慕生物    

研究領域    腫瘤  細胞生物  轉(zhuǎn)運蛋白      

抗體來源    Rabbit    

克隆類型    Polyclonal    

交叉反應    Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,     

產(chǎn)品應用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 內(nèi)質(zhì)網(wǎng)氧化物蛋白Ero1-Lα抗體（石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    52kDa    

細胞定位    細胞漿 細胞膜     

性    狀    Lyophilized or Liquid    

濃    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human ERO1L    

亞    型    IgG    

純化方法    affinity purified by Protein A    

儲 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

內(nèi)質(zhì)網(wǎng)氧化物蛋白Ero1-Lα抗體產(chǎn)品介紹    background:

Ero1-La is an essential oxidoreductase that oxidizes proteins and is required for the folding of immunoglobulins. Ero-1La covalently binds with PDI (protein disulfide-isomerase) and together they produce disulfide bonds between proteins in the endoplasmic reticulum. Ero1-La and SIRT1 regulate adiponectin secretion from adipose tissue. Ero1-La and associated proteins also modulate PPARg (peroxisome proliferator-activated receptor g) and SIRT1 activities. Ero1-La is stimulated by hypoxia, suggesting that it is regulated through the HIF (hypoxia inducible transcrip-tion factor) pathway. Ero1-La is ubiquitously expressed at low levels but expressed at high levels in upper digestive tract and esophagus. Ero1-La may function both as a monomer and a homodimer.

Function:
Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.

Subunit:
Predominantly monomer. May function both as a monomer and a homodimer. Interacts with PDILT.

Subcellular Location:
Endoplasmic reticulum membrane. The association with ERP44 is essential for its retention in the endoplasmic reticulum.

Tissue Specificity:
Widely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum.

Post-translational modifications:
N-glycosylated.
The Cys-94/Cys-99 and Cys-394/Cys-397 disulfide bonds constitute the redox-active center.
The Cys-94/Cys-99 disulfide bond may accept electron from P4HB and funnel them to the active site disulfide Cys-394/Cys-397.

Similarity:
Belongs to the EROs family.

Database links:
UniProtKB/Swiss-Prot: Q96HE7.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.