文獻(xiàn)：泛素交聯(lián)酶抗體應(yīng)用方法

中文名稱    泛素交聯(lián)酶抗體    

英文名稱    E2EPF    

別    名    E2 EPF; E2EPF; EPF5; UBE2S; Ubiquitin carrier protein S; Ubiquitin conjugating enzyme E2 24 kD; Ubiquitin conjugating enzyme E2S; Ubiquitin protein ligase S; AA409170; MGC101982; UBE2S_HUMAN.    

供 應(yīng) 商    遠(yuǎn)慕生物  

研究領(lǐng)域    免疫學(xué)  轉(zhuǎn)錄調(diào)節(jié)因子      

抗體來源    Rabbit    

克隆類型    Polyclonal    

交叉反應(yīng)    Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,     

產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 泛素交聯(lián)酶抗體（石蠟切片需做抗原修復(fù)） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    24kDa    

性    狀    Lyophilized or Liquid    

濃    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human E2EPF    

亞    型    IgG    

純化方法    affinity purified by Protein A    

儲(chǔ) 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

泛素交聯(lián)酶抗體產(chǎn)品介紹    background:

This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq, Jul 2008]

Function:
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.

Subunit:
Component of the APC/C complex. Interacts with CDC20, FZR1/CDH1 and VHL.

Post-translational modifications:
Autoubiquitinated by the APC/C complex during G1, leading to its degradation by the proteasome.

Similarity:
Belongs to the ubiquitin-conjugating enzyme family.

Gene ID:
27338

Database links:

Entrez Gene: 617703 Cow

Entrez Gene: 27338 Human

Entrez Gene: 77891 Mouse

Entrez Gene: 292588 Rat

GenBank: NP_055316.2 Human

Omim: 610309 Human

SwissProt: Q1RML1 Cow

SwissProt: Q16763 Human

SwissProt: Q921J4 Mouse

SwissProt: B5DFI8 Rat

Unigene: 396393 Human

Unigene: 389787 Mouse

Unigene: 120398 Rat


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.