索引：MYC啟動子結(jié)合蛋白1抗體應(yīng)用方法

英文名稱    ENO1    

中文名稱    MYC啟動子結(jié)合蛋白1抗體    

別    名    2 phospho D glycerate hydro lyase; Alpha enolase 1; Alpha enolase; C myc promoter binding protein; EC 4.2.1.11; ENO1L1; Enolase 1 (alpha); Enolase 1 (alpha) like 1; Enolase 1; MBP 1; MBP1; MBPB1; MPB 1; MPB1; MYC promoter binding protein 1; NNE; Non neural enolase; Phosphopyruvate hydratase; Plasminogen binding protein; PPH; Non Neuronal Enolase.    

說 明 書    0.2ml      

研究領(lǐng)域    腫瘤  細(xì)胞生物  免疫學(xué)  神經(jīng)生物學(xué)  結(jié)合蛋白      

抗體來源    Rabbit    

克隆類型    Polyclonal    

交叉反應(yīng)    Human, Mouse, Rat, Cow, Rabbit,     

產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 MYC啟動子結(jié)合蛋白1抗體（石蠟切片需做抗原修復(fù)） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    47kDa    

細(xì)胞定位    細(xì)胞核 細(xì)胞漿 細(xì)胞膜     

性    狀    Lyophilized or Liquid    

濃    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human ENO1    

亞    型    IgG    

純化方法    affinity purified by Protein A    

儲 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

MYC啟動子結(jié)合蛋白1抗體產(chǎn)品介紹    background:

ENO1 encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.

Function:
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.

Subunit:
Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. ENO1 interacts with PLG in the neuronal plasma membrane and promotes its activation. The C-terminal lysine is required for this binding.

Subcellular Location:
Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Isoform MBP-1: Nucleus.

Tissue Specificity:
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

Similarity:
Belongs to the enolase family.

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.