轉(zhuǎn)錄因子ERF抗體應(yīng)用方法

中文名稱    轉(zhuǎn)錄因子ERF抗體    

英文名稱    ERF    

別    名    ERF; ERF_HUMAN; ETS domain containing transcription factor ERF; ETS domain-containing transcription factor ERF; Ets2 repressor factor; PE 2; PE-2; PE2.      

供 應(yīng) 商    遠(yuǎn)慕生物

研究領(lǐng)域    細(xì)胞生物  轉(zhuǎn)錄調(diào)節(jié)因子  表觀遺傳學(xué)      

抗體來(lái)源    Rabbit    

克隆類型    Polyclonal    

交叉反應(yīng)    Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,     

產(chǎn)品應(yīng)用    WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    59kDa    

細(xì)胞定位    細(xì)胞核     

性    狀    Lyophilized or Liquid    

濃    度    1mg/ml    

免 疫 原    KLH conjugated synthetic peptide derived from human ERF/Ets2 Repressor Factor:65-160/548     

亞    型    IgG    

純化方法    affinity purified by Protein A    

儲(chǔ) 存 液    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.    

保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

PubMed    PubMed    

產(chǎn)品介紹    ABT1 (activator of basal transcription 1) is a nuclear protein that associates with the TATA-binding protein (TBP) and enhances basal transcription activity of class II promoters. ABT1 associates with TBP in HeLa nuclear extracts in vitro. Another protein, designated ERF, is a member of the Ets family of transcription factors. The members of the Ets family are grouped because they share a highly conserved DNA binding domain. These factors are involved in growth factor pathways and regulate both proliferation and differentiation. ERF (Ets-2 repressor factor) is a ubiquitously expressed Ets-domain protein that exhibits strong transcriptional repressor activity, suppresses Ets-induced transformation and is regulated by MAPK phosphorylation. ERF transcription may be regulated by Ets-domain proteins. Additionally, modulation of ERF activity is involved in the transcriptional regulation of genes activated during entry into G1 phase.