轉(zhuǎn)錄調(diào)節(jié)因子E2F7抗體實(shí)驗(yàn)說明

中文名稱    轉(zhuǎn)錄調(diào)節(jié)因子E2F7抗體    

英文名稱    E2F7

別    名    E2F 7; E2F transcription factor 7; E2F-7; E2f7; E2F7_HUMAN; FLJ12981; Transcription factor E2F7.    

供 應(yīng) 商    遠(yuǎn)慕生物          

研究領(lǐng)域    細(xì)胞生物  免疫學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  表觀遺傳學(xué)      

抗體來源    Rabbit    

克隆類型    Polyclonal    

交叉反應(yīng)    Human, Mouse, Rat, Chicken, Dog, Horse, Rabbit, Sheep,     

產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 轉(zhuǎn)錄調(diào)節(jié)因子E2F7抗體（石蠟切片需做抗原修復(fù)） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    100kDa    

細(xì)胞定位    細(xì)胞核     

性    狀    Lyophilized or Liquid    

濃    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human E2F7    

亞    型    IgG    

純化方法    affinity purified by Protein A    

儲(chǔ) 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

轉(zhuǎn)錄調(diào)節(jié)因子E2F7抗體產(chǎn)品介紹    background:

The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an “active” E2F transcriptional regulatory complex.

Function:
Along with E2F8, inhibitor of E2F-dependent transcription that is important for the control of the E2F1-TP53 apoptotic pathway. Directly represses E2F1 transcription (By similarity). Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession.

Subunit:
Homodimer and heterodimer: mainly forms homodimers and, to a lesser extent, heterodimers with E2F8. Dimerization is important for DNA-binding. Interacts with HIF1A. 

Subcellular Location:
Nucleus.

Similarity:
Belongs to the E2F/DP family.

Database links:
UniProtKB/Swiss-Prot: Q96AV8.3

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.