染色體區(qū)域穩(wěn)定蛋白/核輸出蛋白1抗體應(yīng)用說明

產(chǎn)品名稱：染色體區(qū)域穩(wěn)定蛋白/核輸出蛋白1抗體

英文名稱：anti-exportin 1/crm1

供應(yīng)商：遠(yuǎn)慕生物

靶點(diǎn)：Exportin 1/CRM1

克隆性：多克隆

標(biāo)記物：HRP等

宿主：鼠，牛，馬，兔，羊等

適應(yīng)物種：Human, Mouse, Rat等

亞型：IgG

形態(tài)：液體或固體

交叉反應(yīng)：Human, Mouse, Rat,  (predicted: Pig, )

產(chǎn)品應(yīng)用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）

not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

保存條件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMed PubMed

 

產(chǎn)品介紹：CRM1 (chromosome region maintenance 1 protein homolog) mediates the nuclear export of cellular proteins (cargoes) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, CRM1 binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The direction of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. CRM1 also mediates the nuclear export of the Rev protein of HIV-1 and Rex protein of HTLV-1. It is involved in Rex dimerization, U3 snoRNA transport from Cajal bodies to nucleoli.

CRM1 is also involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. CRM1 is also involved in the regulation NFAT and AP-1.

 

Subunit:

Found in a U snRNA export complex with RNUXA/PHAX, NCBP1, NCBP2, RAN, XPO1 and m7G-capped RNA (By similarity). Component of a nuclear export receptor complex composed of KPNB1, RAN, SNUPN and XPO1. Found in a trimeric export complex with SNUPN, RAN and XPO1. Found in a nuclear export complex with RANBP3 and RAN. Found in a 60S ribosomal subunit export complex with NMD3, RAN, XPO1. Interacts with DDX3X, NMD3, NUPL2, NUP88, NUP214, RANBP3 and TERT. Also found in complex with several viral proteins involved in RNAs' nuclear export like HIV-1 Rev and HTLV-1 Rex. Interacts presumably with influenza A nucleoprotein. Interacts with Epstein-Barr virus BMLF1. Interacts with NEMF (via its N-terminus). Interacts with the monomeric form of BIRC5/survivin deacetylated at 'Lys-129'.

 

Subcellular Location:

Cytoplasm. Nucleus, nucleoplasm. Nucleus, Cajal body. Nucleus, nucleolus. Note=Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm.

 

Tissue Specificity:

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney.

 

Similarity:

Belongs to the exportin family.

Contains 10 HEAT repeats.

Contains 1 importin N-terminal domain.

SWISS:O14980

Gene ID:7514

Database links:

Entrez Gene: 7514 Human

Entrez Gene: 103573 Mouse

Entrez Gene: 85252 Rat

Omim: 602559 Human

SwissProt: O14980 Human

SwissProt: Q6P5F9 Mouse

SwissProt: Q80U96 Rat

Unigene: 370770 Human

Unigene: 217547 Mouse

Unigene: 34087 Rat

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

免疫組化結(jié)果分析：

1、首先要確定免疫組化技術(shù)是否合格，合格的染色結(jié)果應(yīng)該是背景顏色淺淡或無著色，而陽(yáng)性標(biāo)志物清晰和定位明確。

切片中的正常組織可以作為最簡(jiǎn)便的對(duì)照來判斷染色結(jié)果的可靠與否，例如用vimentin抗體，間質(zhì)成纖維細(xì)胞利血管壁平滑肌細(xì)胞胞漿應(yīng)呈陽(yáng)性?？隙殛?yáng)性的對(duì)照組織卻呈陰性，待測(cè)組織的染色結(jié)果也就不可靠；這現(xiàn)象常是由于抗體失效或過度稀釋、組織中抗原嚴(yán)重丟失等。

 

2、不僅要判斷所檢組織中有或無陽(yáng)性標(biāo)記物，還要注意陽(yáng)性信號(hào)的分布部位和形態(tài)特征。

一般說來，陽(yáng)性信號(hào)應(yīng)與被測(cè)抗原所在的部位一致，大部分細(xì)胞抗原位于胞質(zhì)內(nèi)，其中亦有區(qū)別，有的陽(yáng)性顆粒彌散于整個(gè)細(xì)胞的胞質(zhì)，有的則呈斑塊狀局限于胞漿某處。還有些抗原同時(shí)分布于胞質(zhì)和胞膜(如胃腸腺癌細(xì)胞的ema)，或核與胞質(zhì)(如s-100、hmb-45)。同一種抗原在不同類型的細(xì)胞可定位不同，因而分布的特點(diǎn)也有一定的鑒別意義。

 

遠(yuǎn)慕生物公司優(yōu)勢(shì)：

1.擁有一批專業(yè)高效的研發(fā)團(tuán)隊(duì)，專門解決客戶在實(shí)驗(yàn)過程中遇到的各種難題；

2.多年的應(yīng)用經(jīng)驗(yàn)積累；

3. 產(chǎn)品齊全，滿足不同客戶的使用需求；

4.庫(kù)存充足，供貨周期短，能解決客戶的急需問題；

5.我們秉承誠(chéng)信至上、務(wù)實(shí)做事的信條，是客戶值得信賴的合作伙伴。